Fig. 2

Effect of mmu_circ_0005019 knockdown on the proliferation, migration, differentiation into myofibroblasts and expression of collagen in cardiac fibroblasts. A Knockdown of exogenous mmu_circ_0005019 in cardiac fibroblasts were identified by qRT-PCR (Student’s t-test). n = 3. B Growth curves of cardiac fibroblasts after transfection with mmu_circ_0005019 siRNAs or negative control si-NC were examined by CCK-8 assays (Student’s t-test). n = 3. C Migration of cardiac fibroblasts transfection with mmu_circ_0005019 siRNAs or negative control si-NC were determined by transwell assays (Mann–Whitney U test). n = 10. D Relative expression of Acta2, Vim, Cola1 and Col3a1 of cardiac fibroblasts transfection with mmu_circ_0005019 siRNAs or negative control si-NC were determined by qRT-PCR (Student’s t-test and Mann–Whitney U test). For Col1a1 after si-1 transfection, n = 5; for Acta2 after si-1 transfection, n = 4; for the others, n = 3. The results are expressed as the mean ± sem. *si-1 or si-3 vs. si-NC, P < 0.05