Fig. 3

MiR-641 directly bound to MIAT and STIM1 in VSMCs. a, b The putative binding sites of miR-641 for both MIAT and STIM1 were predicted by starbase online database. c–e The targeting relationship between miR-641 and MIAT or STIM1 was proved by dual-luciferase reporter and RIP assays. f The impacts of MIAT overexpression and silencing on miR-641 expression were revealed by qRT-PCR in VSMCs. g The impacts of miR-641 inhibitor and mimic on STIM1 protein expression were determined by qRT-PCR. h MiR-641 and STIM1 expression were detected by qRT-PCR or western blot in ox-LDL-induced VSMCs and control group. *P < 0.05