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Fig. 7 | BMC Cardiovascular Disorders

Fig. 7

From: Circ_0004104 knockdown alleviates oxidized low-density lipoprotein-induced dysfunction in vascular endothelial cells through targeting miR-328-3p/TRIM14 axis in atherosclerosis

Fig. 7

MiR-328-3p overexpression alleviates ox-LDL-induced dysfunction in HUVECs partly through reducing TRIM14 expression. a The overexpression efficiency of TRIM14 plasmid was tested by Western blot assay. This experiment was performed for three times. bg HUVECs were divided into six groups: control, ox-LDL, ox-LDL + miR-NC, ox-LDL + miR-328-3p, ox-LDL + miR-328-3p + pcDNA and ox-LDL + miR-328-3p + TRIM14. b Cell viability in indicated time points was measured by MTT assay. This experiment was performed for three times with six technical repetitions. c Flow cytometry was carried out to analyze cell apoptosis rate in transfected HUVECs. This experiment was performed for three times with three technical repetitions. d Capillary-like network formation assay was performed to show capillary-like structure in six groups. This experiment was performed for three times with three technical repetitions. e The protein levels of Cleaved-casp3 and VEGFA were detected via Western blot assay. This experiment was performed for three times. f The levels of pro-inflammatory cytokines, including TNF-α and IL-1β, were analyzed by ELISA. This experiment was performed for three times with three technical repetitions. g The production of SOD and MDA was evaluated using their corresponding kits. This experiment was performed for three times with three technical repetitions. *P < 0.05, **P < 0.01, ***P < 0.001. Student’s t-test was utilized to analyze the differences in (a), whereas one-way ANOVA was utilized to assess the differences in (bg)

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