Fig. 6

TRIM14 is a downstream target of miR-328-3p in HUVECs. a The interacted sites between miR-328-3p and TRIM14 3′UTR predicted by StarBase database were shown. b The binding relationship between miR-328-3p and TRIM14 was tested using dual-luciferase reporter assay. This experiment was performed for three times with three technical repetitions. c, d The expression of TRIM14 at mRNA and protein levels was measured in HUVECs exposed to ox-LDL or not by RT-qPCR and Western blot assay. RT-qPCR was applied for three times with three technical repetitions, and Western blot assay was conducted for three times. e, f The mRNA and protein expression of TRIM14 was analyzed in HUVECs with the overexpression or knockdown of miR-328-3p by RT-qPCR and Western blot assay. RT-qPCR was applied for three times with three technical repetitions, and Western blot assay was conducted for three times. g, h HUVECs were transfected with si-circ_0004104 alone or together with anti-miR-328-3p. The mRNA and protein levels of TRIM14 were detected by RT-qPCR and Western blot assay. RT-qPCR was applied for three times with three technical repetitions, and Western blot assay was conducted for three times. **P < 0.01, ***P < 0.001. Student’s t-test was utilized to analyze the differences in b–d, whereas one-way ANOVA was utilized to assess the differences in e–h