Fig. 4

Circ_0004104 acts as miR-328-3p sponge in HUVECs. a The miR-328-3p-binding sites in circ_0004104 predicted by StarBase database were shown. b The overexpression efficiency of miR-328-3p was assessed via RT-qPCR. This experiment was performed for three times with three technical repetitions. c Dual-luciferase reporter assay was applied to verify the interaction between circ_0004104 and miR-328-3p as well as their binding sites. This experiment was performed for three times with three technical repetitions. d RIP assay was carried out to test if miR-328-3p was a target of circ_0004104 in HUVECs. This experiment was performed for three times with three technical repetitions. e The target relationship between circ_0004104 and miR-328-3p was tested by RNA-pull down assay. This experiment was performed for three times with three technical repetitions. f The expression of miR-328-3p in ox-LDL-treated HUVECs was measured by RT-qPCR. This experiment was performed for three times with three technical repetitions. g The regulatory relationship between circ_0004104 and miR-328-3p was tested. The expression of miR-328-3p was analyzed in HUVECs transfected with vector, circ_0004104, si-NC or si-circ_0004104 by RT-qPCR. This experiment was performed for three times with three technical repetitions. *P < 0.05, **P < 0.01, ***P < 0.001. Student’s t-test was utilized to analyze the differences in (b–f), whereas one-way ANOVA was utilized to assess the differences in (g)