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Fig. 3 | BMC Cardiovascular Disorders

Fig. 3

From: Circ_0004104 knockdown alleviates oxidized low-density lipoprotein-induced dysfunction in vascular endothelial cells through targeting miR-328-3p/TRIM14 axis in atherosclerosis

Fig. 3

ox-LDL-induced dysfunction is largely alleviated by the silencing of circ_0004104 in HUVECs. a RT-qPCR was performed to evaluate the interference efficiency of si-circ_0004104 in HUVECs. This experiment was performed for three times with three technical repetitions. bh HUVECs were divided into four groups: control, ox-LDL, ox-LDL + si-NC and ox-LDL + si-circ_0004101. b MTT assay was applied to analyze cell viability in different time points. This experiment was performed for three times with six technical repetitions. c The percentage of apoptotic HUVECs was assessed via flow cytometry. This experiment was performed for three times with three technical repetitions. d The ability of tube formation was analyzed through capillary-like network formation assay in vitro. This experiment was performed for three times with three technical repetitions. e, f The protein levels of Cleaved-casp3 and VEGFA were measured by Western blot assay. This experiment was performed for three times. g The concentrations of inflammation-associated cytokines (TNF-α and IL-1β) were detected by ELISA. This experiment was performed for three times with three technical repetitions. h The production of SOD and MDA was assessed using their matching kits. This experiment was performed for three times with three technical repetitions. *P < 0.05, **P < 0.01, ***P < 0.001. Student’s t-test was utilized to analyze the differences in a, whereas one-way ANOVA was utilized to assess the differences in (bh)

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