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Fig. 2 | BMC Cardiovascular Disorders

Fig. 2

From: Circ_0004104 knockdown alleviates oxidized low-density lipoprotein-induced dysfunction in vascular endothelial cells through targeting miR-328-3p/TRIM14 axis in atherosclerosis

Fig. 2

Circ_0004104 overexpression aggravates ox-LDL-induced dysfunction in HUVECs. a RT-qPCR was applied to assess the overexpression efficiency of circ_0004104 ectopic expression plasmid (circ_0004104) in HUVECs. bg HUVECs were transfected with vector or circ_0004104 plasmid for 24 h prior to ox-LDL exposure (100 μg/mL; 24 h). This experiment was performed for three times with three technical repetitions. b MTT assay was conducted to analyze cell viability after 0 h, 24 h or 48 h. This experiment was performed for three times with six technical repetitions. c Cell apoptosis rate was assessed using flow cytometry. This experiment was performed for three times with three technical repetitions. d Capillary-like network formation assay was utilized to show capillary-like structure in four groups. This experiment was performed for three times with three technical repetitions. e Western blot assay was performed to measure the protein levels of apoptosis marker (Cleaved-casp3) and tube formation-associated marker (VEGFA) in HUVECs. This experiment was performed for three times. f ELISA was utilized to assess the levels of pro-inflammatory cytokines (TNF-α and IL-1β) to analyze cell inflammatory response. This experiment was performed for three times with three technical repetitions. g Cell oxidative stress was analyzed through measuring the levels of SOD and MDA using their matching kits. This experiment was performed for three times with three technical repetitions. *P < 0.05, **P < 0.01, ***P < 0.001. Student’s t-test was utilized to analyze the differences in (a), whereas one-way ANOVA was utilized to assess the differences in (bg)

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