Fig. 2

BM-MSCs treatment increased the level of IL-10 and TGF-β1 cytokines and decreased the level of CK and LDH in the serum of MIRI mice. The level of IL-10 (a), TGF-β1 (b), CK (c), and LDH (d) in the serum of mice treated with IgG, IgG + BM-MSCs, PC61, PC61 + BM-MSCs, splenectomy, and splenectomy + BM-MSCs (n = 5 in each group) are shown. Whole blood was collected 72 h after surgically-induced MIRI, from which the serum was separated. IL-10 and TGF-β1 were measured by ELISA, while CK and LDH were measured by enzyme assays. The bars represent the mean value ± standard deviation of target proteins in each indicated group. BM-MSCs, bone marrow-derived mesenchymal stem cells; CK, creatinine; LDH, lactate dehydrogenase; MIRI, myocardial ischemia–reperfusion injury. SEM ± SD, *p < 0.05, **p < 0.01 and ***p < 0.001