Fig. 5

HDAC4 is the target gene of miRNA-200b-3p. a Identification the binding sites of hsa-miR-200b-3p in the HDAC4 3′-UTR (Red). b HUVECs were transfect with NC mimic and miR-200b mimic for 24 h, the mRNA level of HDAC4 in HUVECs were examined by qRT-PCR, and normalized with GAPDH. (n = 4 for each group). c The protein level HDAC4 in HUVECs was examined by western blot, and normalized with β-Actin (n = 3 for each group). Full-length blots/gels are presented in Additional file 4: Figure S3. d HUVECs were transfect with NC/miR-200b mimic with control adenovirus (Con-Ad) or HDAC4 over-expression adenovirus (HDAC4-Ad) for 48 h, the expression of HDAC4 was accessed by qRT-PCR (n = 4 for each group). e Con-Ad or HDAC4-Ad transfected HUVECs were stimulated with ox-LDL for 6 h. The apoptosis rate of HUVECs was accessed by Annexin V and PI staining (n = 4 for each group). Unpaired t test was used for comparison between groups, *p < 0.05