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Table 4 Relative mRNA expressions of the genes of interest in H9c2 cell line

From: Isolated downregulation of HCN2 in ventricles of rats with streptozotocin-induced diabetic cardiomyopathy

  LG-DIF HG-DIF M-DIF NON-DIF
Cardiac gene expression (n) 3 3 3 2
Hcn2 (%, relative to LG-DIF) 1.00 ± 0.02 1.07 ± 0.04 1.02 ± 0.03 1.00 ± 0.03
Hcn4 (%, relative to LG-DIF) 1.00 ± 0.05 0.94 ± 0.02 0.88 ± 0.04 0.51 ± 0.02*#§
Tnnt2 (%, relative to LG-DIF) 1.00 ± 0.02 0.83 ± 0.00* 0.84 ± 0.01*# 0.26 ± 0.01*#§
Glut1 (%, relative to LG-DIF) 1.00 ± 0.08 0.78 ± 0.04* 0.96 ± 0.05* 1.93 ± 0.18*#§
Glut4 (%, relative to LG-DIF) 1.00 ± 0.05 0.72 ± 0.03* 1.29 ± 0.06*# 0.41 ± 0.00*#§
  1. Expression of Hcn channels (Hcn2 and Hcn4), cardiac marker Tnnt2 and glucose transporters (Glut1 and Glut4) in differentiated H9c2 cells; for details see Table 1) in H9c2 cell line. Mean ± SEM, n = 2–3 per group; *P < 0.05 vs LG-DIF, #P < 0.05 vs HG-DIF, §P < 0.05 vs M)
  2. Groups labelling: LG-DIF—differentiated control cells treated with standard 5.5 mM glucose, HG-DIF—hyperglycaemia, differentiated cells treated with 33 mM glucose, M-DIF—mannitol, differentiated cells treated with 5.5 mM glucose and 27.5 mM of mannitol, NON-DIF – cells harvested before the differentiation