Isolated downregulation of HCN2 in ventricles of rats with streptozotocin-induced diabetic cardiomyopathy

Table 1 Primer sequences for RT-qPCR and mature sequences of rat cardiac microRNAs and control U6 snRNA

Primer sequences for RT-qPCR
Gene	RefSeq accession number	Primer sequence (5′–3′)	PCR product length (bp)
B2m	NM_012512.1	Forward: ATGGAGCTCTGAATCATCTGG Reverse: AGAAGATGGTGTGCTCATTGC	105
Hcn2	NM_053684.1	Forward: CTGACACCTACTGTCGCCTC Reverse: TCTTCTTGCCTATGCGGTCC	129
Hcn4	NM_021658.1	Forward: AGTATCCCATGATGCGCAGG Reverse: TTCTTCTTGCCTATGCGGTCC	70
Hprt1	NM_012583.2	Forward: CAGCTTCCTCCTCAGACCGCTTT Reverse: TCACTAATCACGACGCTGGGACTG	150
Kcnd2	NM_031730.2	Forward: CCTGGAGAAAACCACGAACC Reverse: TGCTGGTGACTCCTTGTTGG	131
Kcnh2	NM_053949.1	Forward: GACCTGCTTACTGCCCTCTAC Reverse: GACGTGCATACAGGTTCAGAG	124
Kcnj11	NM_031358.3	Forward: CACACAGCCACGACAGGATA Reverse: CGTCTGAACGGGACCATCAA	117
Kcnj2	NM_017296.1	Forward: AACCGCTACAGCATCGTCTC Reverse: GCACTGTTGTCGGGTATGGA	111
Kcnq1	NM_032073.1	Forward: CTGGTCTGCCTCATCTTCAG Reverse: TCTGTCCCAAAGAACACCAC	110
Myh6	NM_017239.2	Forward: GCCCTTTGACATCCGCACAGAGT Reverse: TCTGCTGCATCACCTGGTCCTCC	152
Myh7	NM_017240.1	Forward: GCGGACATTGCCGAGTCCCAG Reverse: GCTCCAGGTCTCAGGGCTTCACA	133
Ryr2	NM_001191043.1; NM_032078.2	Forward: ACTGCTGGGCTACGGCTAC Reverse: CTGAAGATGCGGAACCTCTC	99
Sdha	NM_130428.1	Forward: GTGTTGCTGTGTCGCTGATC Reverse: AATGACACCACGGCACTCC	142
Slc2a1 (Glut1)	NM_138827.1	Forward: ATTCTCCGTTTCACAGCCCG Reverse: CTCCTCAATTACCTTCTGGGGG	176
Slc2a4 (Glut4)	NM_012751.1	Forward: GACCCGCCCTTTGCACACCA Reverse: TCACTCGCTGCTGAGGGGGT	174
Tnnt2	NM_012676.1	Forward: GACAGGATCGAAAAGCGTCG Reverse: AGCCTTCCTCCTGTTCTCCT	132
Tnni3	NM_017144.2	Forward: GAGCTTCAGGACCTATGCCG Reverse: AACTTGCCACGCAGGTCATA	143

Mature sequences of rat cardiac microRNAs
microRNA name	Nomenclature of mature form	Sequence	Assay ID
miR-1	rno-miR-1-3p	5′-UGGA AUG UAA AGA AGU GUG UAU-3′	002064
miR-133a	rno-miR-133a-3p	5′-UUUG GUC CCC UUC AAC CAG CUG-3′	002246
U6	U6 snRNA	5′ GTGCTCGCTTCGGCAGCACATATAC TAAAATTGGAACGATACAGAGAAGAT TAGCATGGCCCCTGCGCAAGGATGAC ACGCAAATTCGTGAAGCGTTCCATATT TT-3′	001973

Seed microRNAs sequences are underlined, source mirBase.org
B2m: β2-Microglobulin; Hcn2: hyperpolarization activated cyclic nucleotide gated potassium and sodium channel 2; Hcn4: hyperpolarization activated cyclic nucleotide gated potassium and sodium channel 4; Hprt1: hypoxanthine phosphoribosyltransferase 1; Kcnd2: potassium voltage-gated channel subfamily D member 2; Kcnh2: potassium voltage-gated channel subfamily H member 2; Kcnj11: potassium inwardly rectifying channel subfamily J member 11; Kcnj2: potassium inwardly rectifying channel subfamily J member 2; Kcnq1: potassium voltage-gated channel subfamily Q member 1; Myh6: myosin heavy chain 6 (α‐myosin heavy chain); Myh7: myosin heavy chain 7 (β‐myosin heavy chain); Ryr2: ryanodine receptor; 2 Sdha: succinate dehydrogenase complex flavoprotein subunit A; Slc2a1: solute carrier family 2 member 1 (alias Glut1); Slc2a4: solute carrier family 2 member 4 (alias Glut4); Tnnt2: troponin T2, cardiac type; Tnni3: troponin I3, cardiac type;

ISSN: 1471-2261