Fig. 4

Excessive mitochondrial fission and increased Drp1 expression were shown in diabetic rats. a Representative electron microscopic images of mitochondrial ultrastructure of myocardium subjected to ischemia/reperfusion (magnification ×15,000, scale bar: 1µm). More broken and fragmented mitochondria were shown in group DI/R and Dsev. b Representative images of Drp1 protein stained by immunohistochemistry, quantitative analysis of Drp1 protein measured by optical density and Drp1 mRNA level (magnification ×400, scale bar: 50 µm). Brown granules indicate positive expression of Drp1. Five fields of sections from left ventricles in each group were randomly selected. Real Time PCR measurement Drp1 transcripts normalized to β-actin transcript was conducted on samples extracted from the left ventricles of rats. n = 5 per group.*P: 0.001 vs. S group; ^#P: 0.001 vs. I/R group