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Table 1 PCR and pyrosequencing primer design

From: Effect of ABCA1 promoter methylation on premature coronary artery disease and its relationship with inflammation

Amplification locus Primer sequence Length (bp)
ABCA1-A (8 CpGs) F: 5′-GGGTGGAGGGTATAGTAGGT-3′ 161
R: 5′-AACAAATTCCACTAATACCCTTAACT-3′
Seq: 5′-AACAAATTCCACTAATACCCTTAACT-3′
  1. ABCA1, ATP-binding cassette transporter A1