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Table 1 PCR and pyrosequencing primer design

From: Effect of ABCA1 promoter methylation on premature coronary artery disease and its relationship with inflammation

Amplification locus

Primer sequence

Length (bp)

ABCA1-A (8 CpGs)

F: 5′-GGGTGGAGGGTATAGTAGGT-3′

161

R: 5′-AACAAATTCCACTAATACCCTTAACT-3′

Seq: 5′-AACAAATTCCACTAATACCCTTAACT-3′

  1. ABCA1, ATP-binding cassette transporter A1