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Table 2 Primer sequences, mixes, and their specificities in PCR-SSP assay

From: Association of human platelet alloantigens encoding gene polymorphisms with the risk of Coronary artery disease in Iranian patients

Primers

Sequences

Annealing temperature (°C)

Primer mixes and concentrations

Amplicon size (bp)

HPA-1a

HPA-1b

common

5ʹ-ACTTACAGGCCCTGCCTCT-3ʹ

5ʹ-ACTTACAGGCCCTGCCTCC-3ʹ

5ʹ-AGCCGGAGTGCAATCCTCTG-3ʹ

62

64

66

HPA-1a + common, 0.5 µM

HPA-1b + common, 0.5 µM

196

HPA-2a

HPA-2b

common

5ʹ-CCCCCAGGGCTCCTGAC-3ʹ

5ʹ-GCCCCCAGGGCTCCTGAT-3ʹ

5ʹ-GCCAGCGACGAAAATAGAGG-3ʹ

64

62

62

HPA-2a + common, 0.5 µM

HPA-2b + common, 0.5 µM

241

HPA-3a

HPA-3b

common

5ʹ-GGGGGAGGGGCTGGGGA-3ʹ

5ʹ-GGGGGAGGGGCTGGGGC-3ʹ

5ʹ-GACCTGCTCTACATCCTGGA-3ʹ

64

66

60

HPA-3a + common, 0.5 µM

HPA-3b + common, 0.5 µM

230

HPA-4a

HPA-4b

common

5ʹ-GCTGGCCACCCAGATGCG-3ʹ

5ʹ-AGCTGGCCACCCAGATGCA-3ʹ

5ʹ-GCTGTCCTGGCGTCTGGAG-3ʹ

62

60

62

HPA-4a + common, 0.5 µM

HPA-4b + common, 0.5 µM

158

HPA-5a

HPA-5b

common

5ʹ-AGTCTACCTGTTTACTATCAAAG -3ʹ

5ʹ-AGTCTACCTGTTTACTATCAAAA -3ʹ

5ʹ-CTCTCATGGAAAATGGCAGTA-3ʹ

62

60

62

HPA-5a + common, 2 µM

HPA-5b + common, 2 µM

249

HPA-6a

HPA-6b

common

5ʹ-GACGAGTGCAGCCCCCG-3ʹ

5ʹ-GGACGAGTGCAGCCCCCA-3ʹ

5ʹ-TAGCGGACACAGGAGAAGTC-3ʹ

60

62

62

HPA-6a + common, 0.5 µM

HPA-6b + common, 0.5 µM

163

HPA-9a

HPA-9b

common

5ʹ-GGGCAGCCCCCAGTCCAC-3ʹ

5ʹ-GGGCAGCCCCCAGTCCAT-3ʹ

5ʹ-GACCTGCTCTACATCCTGGA-3ʹ

64

62

62

HPA-9a + common, 0.5 µM

HPA-9b + common, 0.5 µM

212

HPA-15a

HPA-15b

common

5ʹ-TTCAAATTCTTGGTAAATCCTGT -3ʹ

5ʹ-TTCAAATTCTTGGTAAATCCTGG -3ʹ

5ʹ-ATGACCTTATGATGACCTATTC-3ʹ

60

62

60

HPA-15a + common, 2 µM

HPA-15b + common, 2 µM

225

HGH-F

HGH-R

5ʹ-GCCTTCCCAACCATTCCCTTA-3ʹ

5ʹ-TCACGGATTTCTGTTGTGTTTC-3ʹ

64

62

HGH (F + R), 0.2 µM

429

DRα-F

DRα-R

5ʹ-GAGGTAACTGTGCTCACGAACAGC-3ʹ

5ʹ-CACGTTCTCTGTAGTCTCTGGG-3ʹ

74

68

DRα (F + R), 0.2 µM for HPA-5

DRα (F + R), 0.1 µM for HPA-15

607