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Table 2 Primer sequences, mixes, and their specificities in PCR-SSP assay

From: Association of human platelet alloantigens encoding gene polymorphisms with the risk of Coronary artery disease in Iranian patients

Primers Sequences Annealing temperature (°C) Primer mixes and concentrations Amplicon size (bp)
HPA-1a
HPA-1b
common
5ʹ-ACTTACAGGCCCTGCCTCT-3ʹ
5ʹ-ACTTACAGGCCCTGCCTCC-3ʹ
5ʹ-AGCCGGAGTGCAATCCTCTG-3ʹ
62
64
66
HPA-1a + common, 0.5 µM
HPA-1b + common, 0.5 µM
196
HPA-2a
HPA-2b
common
5ʹ-CCCCCAGGGCTCCTGAC-3ʹ
5ʹ-GCCCCCAGGGCTCCTGAT-3ʹ
5ʹ-GCCAGCGACGAAAATAGAGG-3ʹ
64
62
62
HPA-2a + common, 0.5 µM
HPA-2b + common, 0.5 µM
241
HPA-3a
HPA-3b
common
5ʹ-GGGGGAGGGGCTGGGGA-3ʹ
5ʹ-GGGGGAGGGGCTGGGGC-3ʹ
5ʹ-GACCTGCTCTACATCCTGGA-3ʹ
64
66
60
HPA-3a + common, 0.5 µM
HPA-3b + common, 0.5 µM
230
HPA-4a
HPA-4b
common
5ʹ-GCTGGCCACCCAGATGCG-3ʹ
5ʹ-AGCTGGCCACCCAGATGCA-3ʹ
5ʹ-GCTGTCCTGGCGTCTGGAG-3ʹ
62
60
62
HPA-4a + common, 0.5 µM
HPA-4b + common, 0.5 µM
158
HPA-5a
HPA-5b
common
5ʹ-AGTCTACCTGTTTACTATCAAAG -3ʹ
5ʹ-AGTCTACCTGTTTACTATCAAAA -3ʹ
5ʹ-CTCTCATGGAAAATGGCAGTA-3ʹ
62
60
62
HPA-5a + common, 2 µM
HPA-5b + common, 2 µM
249
HPA-6a
HPA-6b
common
5ʹ-GACGAGTGCAGCCCCCG-3ʹ
5ʹ-GGACGAGTGCAGCCCCCA-3ʹ
5ʹ-TAGCGGACACAGGAGAAGTC-3ʹ
60
62
62
HPA-6a + common, 0.5 µM
HPA-6b + common, 0.5 µM
163
HPA-9a
HPA-9b
common
5ʹ-GGGCAGCCCCCAGTCCAC-3ʹ
5ʹ-GGGCAGCCCCCAGTCCAT-3ʹ
5ʹ-GACCTGCTCTACATCCTGGA-3ʹ
64
62
62
HPA-9a + common, 0.5 µM
HPA-9b + common, 0.5 µM
212
HPA-15a
HPA-15b
common
5ʹ-TTCAAATTCTTGGTAAATCCTGT -3ʹ
5ʹ-TTCAAATTCTTGGTAAATCCTGG -3ʹ
5ʹ-ATGACCTTATGATGACCTATTC-3ʹ
60
62
60
HPA-15a + common, 2 µM
HPA-15b + common, 2 µM
225
HGH-F
HGH-R
5ʹ-GCCTTCCCAACCATTCCCTTA-3ʹ
5ʹ-TCACGGATTTCTGTTGTGTTTC-3ʹ
64
62
HGH (F + R), 0.2 µM 429
DRα-F
DRα-R
5ʹ-GAGGTAACTGTGCTCACGAACAGC-3ʹ
5ʹ-CACGTTCTCTGTAGTCTCTGGG-3ʹ
74
68
DRα (F + R), 0.2 µM for HPA-5
DRα (F + R), 0.1 µM for HPA-15
607