Fig. 3
An in vitro coronary circulation system. Three conditions were created as (a–c). a Basal condition, in which both forward and backward flows existed. Resister was used to control the ratio of forward and backward flow, and the pressure and blood flow were stabilized using the Windkessel model. b Only forward condition, in which backward flow was blocked. By adjusting the height of the reservoir, the Pstatic was controlled. High Pstatic is assumed to be pre-hyperemia and low Pstatic is assumed to be hyperemia. c Only backward flow condition, in which forward flow was blocked for measuring