Fig. 6

MiR-206 overexpression-mediated influences in ox-LDL-induced THP-1-derived macrophages are partly overturned by ASPH accumulation. a and b THP-1 cells (n = 3) were transfected with miR-NC, miR-206, miR-206 + pcDNA-NC or miR-206 + pcDNA-ASPH. qRT-PCR and Western blot assay were used to detect the mRNA and protein expression of ASPH. The protein bands shown in Fig. 6B have been cropped. The original Western blot data in Fig. 6B could be find in Additional file 2. The results were assessed by ANOVA followed by Tukey’s test. c-j THP-1 cells were treated with ox-LDL, ox-LDL + miR-NC, ox-LDL + miR-206, ox-LDL + miR-206 + pcDNA-NC or ox-LDL + miR-206 + pcDNA-ASPH. The results in c-j were assessed by ANOVA followed by Tukey’s test. c MTT assay was conducted to assess the cell viability of transfected THP-1 cells (n = 3). d The apoptosis of THP-1 cells (n = 3) was measured by flow cytometry. (E and F) ELISA kits were used to detect the concentrations of TNF-α and IL-6 (n = 3). g-j The levels of iNOS, ROS, SOD and MDA were detected by their matching kits (n = 3). *: P < 0.05