Fig. 4

Si-circTM7SF3-mediated effects in ox-LDL-induced THP-1-derived macrophages are partly counteracted by the addition of anti-miR-206. a THP-1 cells (n = 3) were transfected with si-circTM7SF3 alone or co-transfected with si-circTM7SF3 and anti-miR-206. MiR-206 expression was detected in THP-1 cells by qRT-PCR. The results were assessed by ANOVA followed by Tukey’s test. b-i THP-1 cells were divided into six groups: Control, ox-LDL, ox-LDL + si-NC, ox-LDL + si-circTM7SF3, ox-LDL + si-circTM7SF3 + anti-miR-NC or ox-LDL + si-circTM7SF3 + anti-miR-206. The results in B-I were assessed by ANOVA followed by Tukey’s test. b Cell viability was analyzed by MTT assay (n = 3). c The apoptotic rate was measured by flow cytometry (n = 3). d and e The levels of inflammation-related cytokines were detected by ELISA kits (n = 3). (F-I) The levels of iNOS, ROS, SOD and MDA in THP-1 cells were measured by their corresponding kits (n = 3). *: P < 0.05