Fig. 2

ox-LDL suppresses the viability and promotes the apoptosis, inflammation and oxidative stress of THP-1-derived macrophages through elevating circTM7SF3 level. a CircTM7SF3 level was examined in THP-1 cells (n = 3) transfected with three siRNAs targeting circTM7SF3 by qRT-PCR. Si-circTM7SF3_3 was written as si-circTM7SF3 in the later assays. The results were assessed by ANOVA followed by Tukey’s test. (B-I) THP-1 cells were incorporated into four groups: Control, ox-LDL, ox-LDL + si-NC and ox-LDL + si-circTM7SF3. The results in B-I were assessed by ANOVA followed by Tukey’s test. b Cell viability was assessed using MTT assay (n = 3). c Cell apoptosis was evaluated by flow cytometry (n = 3). d and e Inflammatory response was detected through examining the production of TNF-α and IL-6 via ELISA kits (n = 3). f-i The oxidative stress was analyzed through measuring iNOS, ROS, SOD and MDA by their corresponding kits (n = 3). *: P < 0.05