Fig. 1

CircTM7SF3 level is enhanced in THP-1-derived macrophages by ox-LDL treatment. a The five most up-regulated and down-regulated circRNAs in THP-1-derived macrophages after ox-LDL treatment according to GSE107522 dataset were shown. b The gene information of circTM7SF3 from Circular RNA Interactome (https://circinteractome.nia.nih.gov) was shown. c CircTM7SF3 expression in the serum of Control patients (n = 39) and AS patients (n = 39) was detected by qRT-PCR. The results were analyzed using Student’s t-test. d THP-1 cells (n = 3) were exposed to increased doses of ox-LDL for 24 h, and TM7SF3 level was detected by qRT-PCR. The results were assessed by ANOVA followed by Tukey’s test. e qRT-PCR was applied to measure the enrichment of TM7SF3 in THP-1 cells (n = 3) treated with 50 μg/mL ox-LDL for fixed time interval. The results were assessed by ANOVA followed by Tukey’s test. f After treating with Actinomycin D for 0 h, 4 h, 8 h, 12 h or 24 h, RNA was isolated from THP-1 cells (n = 3), and the expression of circTM7SF3 and TM7SF3 mRNA was detected by qRT-PCR. The results were analyzed using Student’s t-test. g RNA samples isolated from THP-1 cells (n = 3) were grouped into Mock group and RNase R group, and the levels of circTM7SF3 and TM7SF3 mRNA were analyzed by qRT-PCR. The results were analyzed using Student’s t-test. *: P < 0.05