Fig. 5
From: Label-free optical biomarkers detect early calcific aortic valve disease in a wild-type mouse model
Assessment of TPEF Autofluorescence Ratios via TPEF imaging. a Representative TPEF autofluorescence ratio maps for aortic valves of control and pro-calcific mice at 4 and 16 weeks. TPEF 755–860 ratio for b leaflets, c commissures and the d root of control and pro-calcific mice at 4 and 16 weeks. TPEF Col-Cal ratio for e leaflets, f commissures and the g root of control and pro-calcific mice at 4 and 16 weeks. Scale bars—100 µm. N = 3–4. *p < 0.05. Two-way ANOVA with Tukey HSD post hoc test