Fig. 1

Lipid and calcium Deposition assessed by Histology. a–d Representative Hematoxylin and Eosin stained transverse sections at 10 ×, and c, d 20 × depicting plaque-like structures near the commissural walls in pro-calcific mice as marked by arrows. e–h Representative Oil Red O stained transverse sections at 10 ×, and g, h 20 × depicting lipid deposition at the commissural walls in pro-calcific mice as marked by arrows. i–l Representative Alizarin Red S (ARS) stained transverse sections at 10 ×, and k, l 20 × depicting calcium deposition at the commissural walls in pro-calcific mice at 16 weeks, as marked by arrows. Scale Bars—500 µm. N = 3 (mouse)