Table 1 The primer sequences applied in our study
Gene | Tm (°C) | Forward sequence | Reverse sequence |
|---|---|---|---|
TGF-β1 | 62.5 | ATGTCTCAGCCTCTTCTCATTC | TGGTGAATGACAGTGCGGTTATGG |
TNF-α | 58.2 | ATGTCTCAGCCTCTTCTCATTC | GCTTGTCACTCGAATTTTGAGA |
IL-6 | 60.5 | CTCCCAACAGACCTGTCTATAC | CCATTGCACAACTCTTTTCTCA |
IL-1β | 60.5 | TCGCAGCAGCACATCAACAAGAG | TGCTCATGTCCTCATCCTGGAAGG |
BAFF | 53.9 | AACAAGATGTAGACCTCTCAGC | CTGCAGACAGTCTTGAATGATG |
β-actin | 60.5 | CTACCTCATGAAGATCCTGACC | CACAGCTTCTCTTTGATGTCAC |
COL1A1 | 54.3 | AAAGATGGACTCAACGGTCTC | CATCGTGAGCCTTCTCTTGAG |
COL3A1 | 52.4 | GAAAGAATGGGGAGACTGGAC | TACCAGGTATGCCTTGTAATCC |
TIMP-1 | 54.2 | CTCCAGTTTGCAAGGGATAGAT | CAAAGACCTGAAAACCTCCAAC |
MMP-9 | 53.9 | CAAAGACCTGAAAACCTCCAAC | GACTGCTTCTCTCCCATCATC |