Skip to main content
Fig. 4 | BMC Cardiovascular Disorders

Fig. 4

From: microRNA-130a-5p suppresses myocardial ischemia reperfusion injury by downregulating the HMGB2/NF-κB axis

Fig. 4

miR-130a-5p targets HMGB2. a TargetScan identified the binding site between miR-130a-5p and HMGB2 3′ untranslated region. b Dual-luciferase reporter gene assay verified the targeting relation between miR-130a-5p and HMGB2. c, d RT-qPCR and western blot analysis indicated that mRNA expression and protein level of HMGB2 in MIRI mice were both lowered by overexpressed miR-130a-5p, n = 4. The images of d shown are cropped. Two-way ANOVA was used to determine data in panel b and one-way ANOVA was employed to determine data in panels c, d. Tukey’s multiple comparisons test was applied for post hoc test. **p < 0.01; ##p < 0.01. miR microRNA, HMGB high mobility group box, RT-qPCR reverse transcription quantitative polymerase chain reaction, MIRI myocardial ischemia reperfusion injury, ANOVA analysis of variance

Back to article page