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Fig. 2 | BMC Cardiovascular Disorders

Fig. 2

From: microRNA-130a-5p suppresses myocardial ischemia reperfusion injury by downregulating the HMGB2/NF-κB axis

Fig. 2

Overexpressed miR-130a-5p alleviates MIRI in mice. a RT-qPCR showed that the MIRI mice had a lower miR-130a-5p expression pattern, which was increased after overexpressing miR-130a-5p, n = 4. b, c The cardiac function and hemodynamics in mice were detected, n = 10. d ELISA results suggested notable increases in serum CK and cTnT levels from the MIRI mice, which were repressed by overexpressed miR-130a-5p, n = 10. e HE staining revealed that the disrupted myocardial tissues were improved with overexpressed miR-130a-5p, × 200, n = 6. f TUNEL assay revealed that the high apoptosis rates in cardiomyocyte in MIRI mice was declined by overexpressing miR-130a-5p, × 400, n = 6. g Western blot analysis demonstrated that the growth of Bax expression pattern and slowdown of Bcl-2 expression pattern in mice caused by MIRI were reversed by miR-130a-5p overexpression, n = 4. The images of g shown are cropped. Two-way ANOVA was used to determine data in panels c, g, and one-way ANOVA was employed to determine data in panels a, c, d, f. Tukey’s multiple comparisons test was applied for the post hoc test. **p < 0.01; ##p < 0.01. miR microRNA, MIRI myocardial ischemia reperfusion injury, RT-qPCR reverse transcription quantitative polymerase chain reaction, CK creatine kinase, cTnT T Cardiac troponin T, HE hematoxylin and eosin, TUNEL terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling, Bcl2 B-cell lymphoma-2, Bax Bcl2-Associated X, ANOVA analysis of variance

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