Fig. 5

L-T4 treatment promoted the expression of cardiac development proteins in the offspring of SCH pregnant rats. a-b Expression levels of Gata4 and Nkx2–5 mRNA in the offspring of SCH pregnant rats; c-d Representative Western blot images and quantitative analysis of Gata4 and Nkx2–5 expression levels; e-f Representative immunohistochemical images of Gata4 and Nkx2–5 expression and quantitative analysis of Gata4- and Nkx2–5-positive cells in each group (Scale bar = 50 μm). All experiments were repeated at least three times. Data were expressed as the mean ± SEM (n = 5 per group). *p < 0.05 vs Sham group; ^#p < 0.05 vs SCH group