Table 1 Candidate gene polymorphisms, their primer sequence and restriction digestion products
SNP No. | Gene polymorphism | Primer sequence | Amplicon (bp) | Restriction enzymes | Genotypes |
|---|---|---|---|---|---|
SNP 1 | ESR1 IVS1–397 T/C (rs2234693) | 5′-CTG CCA CCC TAT CTG TAT CTT TTC CTA TTC TCC- 3′ (F) 5′-TCT TTC TCT GCC ACC CTG GCG TCG ATT ATC TGA- 3′ (R) | 1372 | PvuII | TT = 982 & 390 bp TC = 982, 390 & 1372 bp CC = 1372 bp |
SNP 2 | ESR1- IVS1–351 A/G (rs9340799) | 5′-CTG CCA CCC TAT CTG TAT CTT TTC CTA TTC TCC- 3′ (F) 5′-TCT TTC TCT GCC ACC CTG GCG TCG ATT ATC TGA- 3′ (R) | 1372 | XbaI | AA = 936 & 436 bp AG = 936, 436 & 1372 bp GG = 1372 bp |
SNP 3 | CETP-TaqIB (C277T) (rs708272) | 5′-CAC TAG CCC AGA GAG AGG AGT GCC-3′ (F) 5′-CTG AGC CCA GCC GCA CAC TAA C-3′ (R) | 535 | TaqI | B2B2 = 535 bp B1B2 = 535, 361 & 174 bp B1B1 = 361 & 174 bp |
SNP 4 | MTHFR C677T (rs1801133) | 5′-TGA AGG AGA AGG TGT CTG CGG GA-3′ (F) 5′-AGG ACG GTG CGG TGA GAG TG-3′ (R) | 198 | HinfI | CC = 198 bp CT = 198, 175 & 23 bp TT = 175 & 23 bp |
SNP 5 | MTHFR G1793A (rs2274976) | 5′-CTC TGT GTG TGT GTG CAT GTG TGC G-3′ (F) 5′-GGG ACA GGA GTG GCT CCA ACG CAG G-3′ (R) | 310 | BsrbI | GG = 233 & 77 bp GA = 310, 233 & 77 bp AA = 310 bp |
SNP 6 | MS A2756G (rs185087) | 5′- TGT TCC AGA CAG TTA GAT GAA AAT C-3′ (F) 5′- GAT CCA AAG CCT TTT ACA CTC CTC-3′ (R) | 211 | HaeIII | AA = 211 bp AG = 211, 131 & 80 bp GG = 131 & 80 bp |