Fig. 5

Effect of RAGE silencing on Kv1.2/1.5 expression in CSMCs exposed to AGE. The CSMCs were transfected with RAGE siRNA and the effects of AGE on Kv1.2 and Kv1.5 expression after silencing of RAGE were detected by Western blot analyses. a Representative blots showing the efficiency of RAGE silencing. Scr, scrambled siRNA (negative control); siR 1 and siR 2, two different sequences of RAGE siRNA. b Mean data for RAGE expression levels in CSMCs with or without RAGE silencing. c Representative blots showing protein expression of RAGE, Kv1.2 and Kv1.5 in CSMCs treated with Scr siRNA, 100 μg/mL AGE + Scr siRNA, RAGE siRNA or 100 μg/mL AGE + RAGE siRNA, respectively. The cells were transfected for 48 h and then incubated with or without AGE for 24 h. β-actin was used as an internal reference. d-f Mean data for protein expression levels. n = 3 per group. * P < 0.05 vs. Scr siRNA group; # P < 0.05 vs. AGE + Scr siRNA group