Fig. 4

Protein expressions in cultured CSMCs detected by Western blot. The CSMCs were incubated with DMEM, 100 μg/mL BSA, 100 μg/mL AGE, 100 μg/mL AGE+ 10 μM ALA, 100 μg/mL AGE+ 1 μM PIO, or 100 μg/mL AGE+ 1 μM PIO + 1 μM GW9662 for 24 h. a Representative blots for RAGE, Kv1.2, Kv1.5, PPAR-γ and NOX-2. β-actin was used as an internal reference. b-d Mean data for protein expression levels. n = 3 per group. * P < 0.05 vs. BSA; # P < 0.05 vs. AGE; § P < 0.05 vs. AGE + PIO