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Fig. 3 | BMC Cardiovascular Disorders

Fig. 3

From: Rno-microRNA-30c-5p promotes myocardial ischemia reperfusion injury in rats through activating NF-κB pathway and targeting SIRT1

Fig. 3

SIRT1 was the target gene of rno-miR-30c-5p. a The expression of rno-miR-30c-5p was detected by qRT-PCR (N = 5 each group). b Correlation analysis between the expression of rno-miR-30c-5p and SIRT1. c The mRNA and protein expression of SIRT1 were detected by qRT-PCR and Western blot (N = 3 each group). d The binding site of rno-miR-30c-5p at 3′-UTR of SIRT1 was predicted by TargetScan software. e The interaction between rno-miR-30c-5p and SIRT1 was analyzed by DLR assay. f The interaction between rno-miR-30c-5p and SIRT1 was analyzed by RIP assay. Data were presented as mean ± SD (three independent repetitions for each sample). *P < 0.05, vs. Sham group (a); *P < 0.05, vs. IR and inhibitor NC group, #P < 0.05, vs. IR and mimics NC group (c); *P < 0.05, vs. NC-mimics group (e); *P < 0.05, vs. Anti-IgG group (f)

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