Fig. 2

Rno-miR-30c-5p enhanced the inflammation, promoted the apoptosis, and activated NF-κB pathway in IR myocardial cells. a The mRNA expression of rno-miR-30c-5p was detected by qRT-PCR (N = 3 each group). b The levels of IL-1β, IL-6 and TNF-α were detected by ELISA (N = 3 each group). c The apoptotic index (%) was detected by Flow cytometry (N = 3 each group). d The expression of Bax, Bcl-2 and caspase-3 was detected by Western blot (N = 3 each group). e The expression of p-IκBα, IκBα, p-NF-κB p65 and NF-κB p65 was detected by Western blot (N = 3 each group). Data were presented as mean ± SD (three independent repetitions for each sample). ^*P < 0.05, vs. IR and inhibitor NC group; ^#P < 0.05, vs. IR and mimics NC group