Fig. 3

Omentin-1 inhibited TGF-β1-induced endothelial-mesenchymal transition (EndMT) of human umbilical vein endothelial cells (HUVECs). Vimentin and VE-Cad protein levels in HUVECs were detected via western blotting (a). The mRNA levels of vimentin and VE-Cad were detected via RT-qPCR (b). Expression of mRNA were normalized to GAPDH. Representative images of immunofluorescence staining for vimentin (c) and VE-Cad (d) (200× magnification) in HUVECs. The p-SMAD3 and t-SMAD3 protein levels in HUVECs were detected via western blotting (e). (f) Representative images of HUVECs scratch assay (× 100 magnification). The values were mean ± SEM of three independent experiments. *P < 0.05 vs control group, **P < 0.01 vs control group, ***P < 0.001 vs control group, †P < 0.05 vs TGF-β1 group, ††P < 0.01 vs TGF-β1 group, †††P < 0.001 vs TGF-β1 group