Fig. 5

Immunofluorescent staining examined JUP expression levels with either wild type or mutant DSP. HEK293T cells were transfected with either wild type or mutant plasmids. Blue indicate nuclear (DAPI) and red indicate JUP. a Representative images of the immunofluorescent staining of transfected HEK293T cells; b Confocal microscopic detection of the colocalization of JUP with DAPI; c Colocalization analysis of JUP and DAPI (n = 10). DSP, Desmoplakin; JUP, Junction plakoglobin; WT, wild type