Fig. 4From: Next-generation sequencing identified novel Desmoplakin frame-shift variant in patients with Arrhythmogenic cardiomyopathyJUP and β-catenin expression in cytoplasm and nuclear, separately. HEK293T cells were transfected with either wild type or mutant plasmids. a-b JUP was significantly upregulated and a-c β-catenin was downregulated in cells transfected with mutant type in the nuclear, rather than cytoplasm, when compared with wild type DSP. GAPDH served as an internal control in the cytoplasm and Lamin B served as an internal control in the nuclear. Blank plasmids carrying no DSP gene served as control group. DSP, Desmoplakin; JUP, Junction plakoglobin; WT, wild typeBack to article page