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Table 3 ‘Routine lab parameters and methodology'. HPLC High pressure liquid chromatography, ECLIA Electrochemiluminescenceimmunoassay

From: The Tyrolean early vascular ageing-study (EVA-Tyrol): study protocol for a non-randomized controlled trial

ParameterUnitMethodReagentAnalyzer
Glucosemg/dlHexokinase methodRocheCobas 8000
HbA1c (DCCT/NGSP)%HPLCMenariniHA 8180 T
HbA1c (IFCC)mmol/molHPLCMenariniHA 8180 T
InsulinmU/lECLIARocheCobas 8000
Cholesterolmg/dlEnzymatic color assayRocheCobas 8000
HDL-Cholesterolmg/dlEnzymatic color assayRocheCobas 8000
LDL-Cholesterolmg/dlEnzymatic color assayRocheCobas 8000
Triglyceridemg/dlEnzymatic color assayRocheCobas 8000
Lipoprotein (a)nmol/lParticle-enhanced immunological clouding assayRocheCobas 8000
Total-Homocysteinumol/lChemiluminescence microparticle immunoassayAbbottArchitect
Ureamg/dlKinetic test with urease and Glutamate dehydrogenaseRocheCobas 8000
Creatinine (enzym.-IDMS)mg/dlEnzymatic color assayRocheCobas 8000
Total-proteing/dlBiuret testRocheCobas 8000
Uric acidmg/dlEnzymatic color assay with uricaseRocheCobas 8000
Potassiummmol/lIndirect potentiometryRocheCobas 8000
Calciummmol/lPhotometric with 5-Nitro-5′-methyl-BAPTARocheCobas 8000
GOT (ASAT)U/lAccording to IFCC recommendations, though optimizedRocheCobas 8000
GPT (ALAT)U/lAccording to IFCC recommendations, though optimizedRocheCobas 8000
Gamma-GTU/lEnzymatic color assayRocheCobas 8000
Creatine kinaseU/lEnzymatic UV-AssayRocheCobas 8000
C-reactive proteinmg/dlParticle-enhanced immunological clouding assayRocheCobas 8000
Ferritinug/lParticle-enhanced immunological clouding assayRocheCobas 8000
TSHmU/lECLIARocheCobas 8000
Free thyroxine (FT4)pmol/lECLIARocheCobas 8000
Thyroglobulinug/lECLIARocheCobas 8000
Thyroglobulin-AntibodieskU/lECLIARocheCobas 8000
ESR after 1 hmm/hWestergren-MethodMechatronicsStarrsed Auto Compact
LeukocytesG/lBiofluorescence/flow cytometrySysmexXE-5000
Absolute number of NeutrophilesG/lBiofluorescence/flow cytometrySysmexXE-5000
ErythrocytesT/lImpedance methodSysmexXE-5000
Haemoglobing/lPhotocolorimetricSysmexXE-5000
Hematocritl/lImpedance methodSysmexXE-5000
PlateletsG/lImpedance methodSysmexXE-5000
MCHpgCalculatedSysmexXE-5000
MCHCg/lCalculatedSysmexXE-5000
MCVflCalculatedSysmexXE-5000
Erythrocyte-distributional width%CalculatedSysmexXE-5000
Band neutrophiles%Manual microscopymanualLight microscopy
Segmented neutrophiles%Biofluorescence/flow cytometry and light microscopySysmexXE-5000
Lymphocytes%Biofluorescence/flow cytometry and light microscopySysmexXE-5000
LymphocytesG/lBiofluorescence/flow cytometry and light microscopySysmexXE-5000
Monocytes%Biofluorescence/flow cytometry and light microscopySysmexXE-5000
Eosinophils%Biofluorescence/flow cytometry and light microscopySysmexXE-5000
Basophils%Biofluorescence/flow cytometry and light microscopySysmexXE-5000
Plasma cells%Manual microscopyManualLight microscopy
Folic acidμg/lCompetitive binding assayRocheCobas 8000
Vitamin B12pmol/lECLIARocheCobas 8000