High-dose nitrate therapy recovers the expression of subtypes α1 and β-adrenoceptors and Ang II receptors of the renal cortex in rats with myocardial infarction-induced heart failures

Table 1 Primer subsequence and annealing temperature of α₁, β adrenergic receptor and Angiotensin II receptor subtypes

Receptor	Primer subsequence	Length of amplified products	Annealing temperature
β₁-AR	sense:5′-GGGCAACGTTGGTGATCG-3′	213 bp	58 °C
β₁-AR	antisense:5′-CTGGCCGTCACACATAGCAC-3′	213 bp	58 °C
β₂-AR	sense:5′-GAGACCCTGTGCGTGATTGC-3′	388 bp	58 °C
β₂-AR	antisense: 5′-CCTGCTCCACCTGGCTGAGG-3′	388 bp	58 °C
β₃-AR	sense:5′-AGTGGGACTCCTCGTAATG-3′	444 bp	59 °C
β₃-AR	antisense: 5′-CGCTTAGCTACGAAC-3’	444 bp	59 °C
α_1A-AR	sense:5′-CAAGGCCTCAAGTCCGGCCT-3’	156 bp	58 °C
α_1A-AR	antisense:5′-CTCTCGAGAAAACTTGAGCAG-3’	156 bp	58 °C
α_1B-AR	sense:5′-ATCGTGGCCAAGAGGACCAC-3’	287 bp	62 °C
α_1B-AR	antisense: 5′-CTCTCGAGAAAACTTGAGCAG-3’	287 bp	62 °C
α_1D-AR	sense:5′-CGTGTGCTCCTTCTACCTACC-3’	304 bp	58 °C
α_1D-AR	antisense:5′-GCACAGGACGAAGACACCCAC-3’	304 bp	58 °C
AT₁R	sense: 5′-CGTCATCCATGACTGTAAAATTTC-3’	306 bp	53 °C
AT₁R	antisense: 5′-GGGCATTACATTGCCAGTGTG-3’	306 bp	53 °C
AT₂R	sense: 5′-GTGTGGGCCTCCAAACCATTGCTA-3’	445 bp	61 °C
AT₂R	antisense: 5′-TTGCTGCCACCAGCAGAAAG-3’	445 bp	61 °C
GAPDH	sense:5′-TGCACCACCAACTGCTTAGC-3’	196 bp	57 °C
GAPDH	antisense: 5′-GGCATGGACTGTGGTCATGAG-3’	196 bp	57 °C

AR Adrenergic receptor, ATR Angiotensin II receptor, GAPDH glyceraldehyde-3-phosphate dehydrogenase

ISSN: 1471-2261