Table 1 PCR primers for the human TBX5 gene promoter
PCR primers | Sequences | Location | Position | Products |
|---|---|---|---|---|
Sequencing | ||||
 TBX5-F1 | 5′-GGGTTTGGGAGAAGGATTTC-3′ | 3981 | -1020 bp | 688 bp |
 TBX5-R1 | 5′-GAGGCACGAGGCACTCTATT-3′ | 4668 | -333 bp |  |
 TBX5-F2 | 5′-AGAAATTGTGCCCATTGATC-3′ | 4593 | -408 bp | 677 bp |
 TBX5-R2 | 5′-TCTCCGTCTTCGCCTATCAG-3′ | 5269 | + 269 bp |  |
Functioning | ||||
 TBX5-F | 5′-(KpnI)-CGCTCGGAGTTTCCCCTTTT-3′ | 3877 | -1124 bp | 1294 bp |
 TBX5-R | 5′-(HindIII)-CGGAGGAATGAGGGTGATGAAC −3′ | 5170 | + 170 bp |  |