Heat shock protein 90 is downregulated in calcific aortic valve disease

Table 2 Up- and down-regulated proteins as determined by 2D-DIGE in calcified valves as compared to control valves

				Comparison Control vs. calcified valves^a			Protein identification^c
Spot	Protein	UniProt	Description	Ratio	P-value	Theoretical pI/MW (kDa)^b	Score (MS, MSMS)	SC (P) (MS, MSMS)
1	HSP90α/β	P07900/ P08238	Heat shock protein HSP90AA1/AB1	− 2.05	0.022	AA1: 1: 4.94/84.7	113.0, −	39(18),-
						2: 5.07/98.2
						AB1: 4.96/83.3
2	C9	P02748	Complement 9	1.74	0.014	5.43/63.2 (5.42/60.9)	83.2, −	21(14),-
3				1.68	0.016	(C9a: 4.59/27.8)	88.5, −	18(13),-
4				1.55	0.018	(C9b: 8.63/33.2)	-, 72.9	-, 5(2)
6	PDIA3	P30101	Protein disulfide-isomerase A3	−3.35	0.016	5.98/56.8 (5.61/54.3)	162.0,-	46.8(21),-
8	ANXA2	P07355	Annexin A2	−2.22	0.012	1: 7.57/38.6 (7.56/38.5)	71.1,-	21(5),-
9				− 2.95	0.019	2: 8.53/40.4 (8.54/40.3)	199*,-	33(13),-
10	APCS	P02743	Serum amyloid P-component	2.26	0.014	6.10/25.4 (6.12/23.3)	75*, −	20(4),-
11				1.59	0.039		80*,-	20(5),-
12				1.51	0.046		82*,-	25(5),-
13	TAGLN	Q01995	Transgelin	3.45	0.032	8.87/22.6 (8.88/22.5)	176.00,-	76(27),-
15	LGALS1	P09382	Galectin-1	−2.19	0.012	5.30/14.7 (5.30/14.6)	92.4, 64.8	57(7), 44(2)

^aThe ratio represents the change of the mean normalized volumes. Statistical significance is shown with the t-test (P < 0.05). Ratio and t-test values of the unidentified spots were as follows: spot 5 (−2.60, 0.0200), spot 7 (− 2.91, 0.0311) and spot 14 (− 2.12, 0.0159)
^bThe theoretical expected spot position in the gel according to the full or matured (in brackets) protein sequence is shown. If the protein exists in different isoforms, then the specific number of the isoform is likewise indicated
^cThe protein identification shows the MS and/or MSMS scores based on analysis with the UltrafleXtreme MALDI TOF/TOF instrument or measurements with the VOYAGER-DE™ STR (*). In addition, the sequence coverage (SC) and number of matched peptides (P) are shown

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