Fig. 2
From: Heat shock protein 90 is downregulated in calcific aortic valve disease
HSP90 expression in aortic valves. a Western blot analysis revealed decreased HSP90β protein levels in stenotic valves (AS) when compared to control valves (C). Results are mean ± SD, ** = P < 0.01. Representative Western blots are shown. Immunohistochemical stainings against HSP90α (b, d) and HSP90β (c, e) in aortic valves. VICs in aortic valve displayed cytoplasmic positivity for HSP90α (b) and HSP90β (c) stainings. Representative examples of adjacent sections of the same area of a control valve. Also the the endothelium was strongly positive for HSP90α (d) and HSP90β (e). Representative examples of adjacent sections of the same area of neovasculature in calcified valves. There was also a wide positive reaction in valve interstitial cells (VICs) and patchy positivity in inflammatory cells, mainly small lymphocytes. All pictures are at the same scale, scale bar depicts 100 μm