Fig. 5
Coronary microembolization reduced cardiomyocyte apoptosis via Nrf2/HO-1 signaling approach. The impacts of CME and AAV-Nrf2 on the protein levels of Nrf2 and HO-1 pathways as well as bax, bcl-2, caspase-3 and caspase-9 in the myocardial tissue in rat 6 h post-CME modeling are as above: the conclusions of the Western blotting detection. AAV-Nrf2(CME): rats injected Nrf2 undergone CME operation; AAV-control (CME): rats injected AAV-control undergone CME operation; a the bands of total-Nrf2,HO-1 and b-actin, b the bands of bax,bcl-2 and b-actin, c the bands of cleave-caspase-3,caspase-9 and b-actin, d Nrf2, e bax, f cleave-caspase-3, g HO-1, h bcl-2, i caspase-9. CME: coronary microembolization; Nrf 2: nuclear factor erythroid 2-like; HO-1: Heme oxygenase-1; AAV: adeno-associated virus. Data is shown as Mean ± SD. a: P < 0.05 contrasted with sham; b: P < 0 .05 contrasted with CME or AAV-control (CME)