Table 2 Primer sequences used for Real-Time Quantitative RT-PCR
From: DNA methylation dysregulations in rheumatic heart valve disease
Gene | Primer Sequence |
|---|---|
ICAM-1/F | CCCCGTTGCCTAAAAAGG |
ICAM-1/R | TTGCACATTGCTCAGTTCATACAC |
DNMT1/F | AAGAACGGCATCCTGTACCGAGTT |
DNMT1/R | TGCTGCCTTTGATGTAGTCGGAGT |
DNMT3a/F | TTTGAGTTCTACCGCCTCCTGCAT |
DNMT3a/R | GTGCAGCTGACACTTCTTTGGCAT |
DNMT3b/F | AGTGTGTGAGGAGTCCATTGCTGT |
DNMT3b/R | GCTTCCGCCAATCACCAAGTCAAA |
β-actin/F | GCACCACACCTTCTACAATGAGC |
β-actin/R | GGATAGCACAGCCTGGATAGCAAC |