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Fig. 8 | BMC Cardiovascular Disorders

Fig. 8

From: RhoA/rock signaling mediates peroxynitrite-induced functional impairment of Rat coronary vessels

Fig. 8

Nitrotyrosine-modification of proteins by peroxynitrite. 3-NT-modified proteins were detected by immunoprecipitation. a Representative blots showing detection of 3-NT-modified RhoA, ROCK1, and ROCK2. b Representative blots showing detection of 3-NT-modified Kv1.2 and Kv1.5. c Quantification of 3-NT-modified RhoA, ROCK1, and ROCK2. 5 μM peroxynitrite (but not decomposed peroxynitrite) induced an increase in the levels of 3-NT-modified RhoA, ROCK1, and ROCK2; this was reversed by 100 μM urate (a scavenger of peroxynitrite). d Quantification of 3-NT-modified Kv1.2 and Kv1.5. 5 μM peroxynitrite (but not decomposed peroxynitrite) induced an increase in the levels of 3-NT-modified Kv1.2; this was reversed by 100 μM urate. In contrast, peroxynitrite had no effect on levels of 3-NT-modified Kv1.5. NG: 5.5 mM glucose; ONOO: 5 μM peroxynitrite; DC-ONOO: 5 μM decomposed peroxynitrite; ONOO + Urate: 5 μM peroxynitrite and 100 μM urate (to scavenge peroxynitrite); C3 or ONOO + C3: 5 μM peroxynitrite and C3 transferase (to inhibit RhoA); Y-27632 or ONOO + Y-27632: 5 μM peroxynitrite and Y-27632 (to inhibit ROCK). Data are shown as mean ± SD (n = 5). * P < 0.05 compared with NG; &P < 0.05 compared with ONOO

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