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Fig. 7 | BMC Cardiovascular Disorders

Fig. 7

From: RhoA/rock signaling mediates peroxynitrite-induced functional impairment of Rat coronary vessels

Fig. 7

mRNA and protein expression of Kv1.2 and Kv1.5 in coronary vascular smooth muscle cells. a Kv1.2 mRNA expression (real-time PCR) was not affected by 5 μM peroxynitrite. b Kv1.5 mRNA expression (real-time PCR) was not influenced by 5 μM peroxynitrite. c Representative blots for Kv1.2 and Kv1.5. GAPDH expression was used as an internal reference. d Mean data for protein expression levels. Peroxynitrite (5 μM) had no effect on the protein expression of Kv1.2 and Kv1.5. NG: 5.5 mM glucose; ONOO: 5 μM peroxynitrite; DC-ONOO: 5 μM decomposed peroxynitrite; C3 or ONOO + C3: 5 μM peroxynitrite and C3 transferase (to inhibit RhoA); Y-27632 or ONOO + Y-27632: 5 μM peroxynitrite and Y-27632 (to inhibit ROCK). Data are shown as mean ± SD (n = 5)

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