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Fig. 4 | BMC Cardiovascular Disorders

Fig. 4

From: RhoA/rock signaling mediates peroxynitrite-induced functional impairment of Rat coronary vessels

Fig. 4

mRNA expressions of RhoA, ROCK1, and ROCK2 in rat coronary vascular smooth muscle cells. mRNA expression was determined using real-time PCR. Treatment with high glucose (23 mM D-glucose) resulted in an increase in the mRNA expression of RhoA (a), ROCK1 (b), and ROCK2 (c). However, exogenous peroxynitrite was without significant effect on the mRNA expression of RhoA (d), ROCK1 (e), and ROCK2 (f). NG: 5.5 mM glucose; LG: 5.5 mM D-glucose plus 17.5 mM L-glucose; HG: 23 mM D-glucose; ONOO: 5 μM peroxynitrite; DC-ONOO: 5 μM decomposed peroxynitrite; ONOO + Urate: 5 μM peroxynitrite and 100 μM urate (to scavenge peroxynitrite). Data are shown as mean ± SD (n = 5). * P < 0.05 compared with the NG group

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