Fig. 2

Immunohistochemistry showing protein expressions of RhoA, ROCK1, and ROCK2 in rat coronary small coronary artery rings. Sections were stained with rabbit primary antibodies against RhoA, ROCK1, or ROCK2, followed by goat anti-rabbit biotinylated secondary antibody, and then visualized with diaminobenzidine. Sections were counterstained with hematoxylin and eosin. Brown staining in the image is indicative of expression of the protein of interest. Treatment with high glucose (23 mM D-glucose) was associated with enhanced expressions of RhoA, ROCK1, and ROCK2 compared with normal glucose (5.5 mM). These effects of high glucose were partially reversed by C3 transferase (a RhoA inhibitor) and Y-27632 (a ROCK inhibitor). NG: 5.5 mM glucose; HG: 23 mM D-glucose; HG + C3: 23 mM D-glucose and 1 μg/ml C3 transferase; HG + Y27632: 23 mM D-glucose and 10 μM Y-27632. Magnification: ×200