Fig. 4
From: Wnt signaling contributes to vascular calcification by induction of matrix metalloproteinases
Effects of Wnt antagonism on CM-induced VSMC calcification and MMP transcription in VSMC. VSMC were treated as indicated. Measurements of calcium contents (a) or alkaline phosphatase (ALP) activities (b) in VSMC cultures served as markers for VSMC calcifications (n = 4). Shown are means ± SD. nsnot significant, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001. c VSMC were treated for 24 h with CM with or without the presence of the Wnt antagonist FH535. The effects on mRNA expressions of MMP-2 and MMP-9 were determined by qPCR measurements (n = 3) Shown are means ± SD, nsnot significant, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001