Fig. 3
From: Wnt signaling contributes to vascular calcification by induction of matrix metalloproteinases
Effects of Wnt antagonism on Wnt agonist-induced Wnt activity, calcification and MMP-production in VSMC. a VSMC transfected with TCF/LEF-luciferase reporter plasmids were treated as indicated for 3 days and luciferase activity as an indicator for activation of Wnt signaling was measured. Data were normalized to a parallel transfected renilla reporter vector (n = 3). b VSMC were treated as indicated. Treatment dependent calcium contents of VSMC cultures were determined by the o-cresolphthalein complexone method. (n = 4). c VSMC were treated as indicated and gelatinolytic activities in aliquots of supernatants were determined by conversion of dye-quenched (DQ)-gelatin substrate (n = 3). Shown are means ± SD, **P ≤ 0.01, ***P ≤ 0.001