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Fig. 2 | BMC Cardiovascular Disorders

Fig. 2

From: Wnt signaling contributes to vascular calcification by induction of matrix metalloproteinases

Fig. 2

Effects of Wnt signaling on gelatinase gene expression and secretion and reverse effects of recombinant gelatinases on Wnt signaling. a VSMC were treated for 24 h with the Wnt agonist I and gene expressions of MMP-2 and MMP-9 were determined by qPCR measurements (n = 3). b VSMC were treated as indicated for up to 72 h. Gelatinolytic activities in aliquots of supernatants were determined by conversion of dye-quenched (DQ)-gelatin substrate at indicated time points (n = 4). c VSMC transfected with TCF/LEF-luciferase reporter plasmids were treated as indicated for 3 days and luciferase activity as an indicator for activation of Wnt signaling was measured. Data were normalized to a parallel transfected renilla reporter vector (n = 4). Shown are means ± SD; nsnot significant to control, *P ≤ 0.05 (compared to control), ***P ≤ 0.001 (compared to control)

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