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Table 2 Myoblast and myotubes characteristics in HFrEF patients versus controls

From: Primary skeletal muscle myoblasts from chronic heart failure patients exhibit loss of anti-inflammatory and proliferative activity

Characteristic Controls n = 6 HFrEF n = 6 p-value
Myogenicity (% Desmin positive cells)    
 Myoblasts 92.14 ± 1.76 91.09 ± 1.89 0.608
 Myotubes 90.80 ± 0.43 92.30 ± 1.19 0.485
Growth characteristics    
 Maximum cell index (time in hours) 114.5 ± 12.05 136.5 ± 5.16 0.214
 Cell Index (at 90 h) 1.80 ± 0.35 1.0 ± 0.10 0.032
Differentiation capacity    
 Differentiation Index (DI) 55.49 ± 2.67 46.57 ± 3.29 0.065
 Deformed Myotubes Index (DMI) 1.96 ± 0.36 3.10 ± 0.74 0.394
 Myogenic Fusion Index (MFI) 57.33 ± 2.40 50.41 ± 1.98 0.093
   < 3 Nuclei 20.67 ± 2.45 26.17 ± 2.55 0.266
  3–10 Nuclei 56.50 ± 4.59 50.17 ± 5.89 0.574
   > 10 Nuclei 21.17 ± 4.64 18.83 ± 2.63 0.905
Viability (% alive cells)    
 7-AAD- 97.83 ± 0.51 97.05 ± 0.60 0.476
 Annexin-V- 92.25 ± 1.34 90.59 ± 1.66 0.352
  1. N Number of subjects, HFrEF Heart failure with reduced ejection fraction. Significant p-values are highlighted in bold. Data are expressed as mean ± SEM