|  | Sham | MI-CON | MI-GFP | MI-SiRNA | MI-Sema | MI-PBS |
---|---|---|---|---|---|---|---|
1-RT-PCR (target mRNA /GAPDH) | TH | 1 | 2.93 ± 0.21 | 2.87 ± 0.18 | 3.82 ± 0.34* | 1.84 ± 0.14** | 3.12 ± 0.23*** |
CHAT | 1 | 1.66 ± 0.14 | 1.71 ± 0.17 | 1.67 ± 0.16 | 1.61 ± 0.14**** | 1.72 ± 0.17 | |
2- IHC (um2/mm2) | TH-positive | 1281 ± 75.1 | 3589 ± 278.1 | 3539 ± 176 | 4707 ± 193.2* | 2330 ± 162.4** | 3564 ± 118.9*** |
 | Sema 3A protein | 0.14 ± 0.02 | 0.35 ± 0.03 | 0.33 ± 0.025 | 0.167 ± 0.02* | 0.44 ± 0.03** | 0.31 ± 0.02*** |
3- WB (target protein/GAPDH) | TH protein | 0.19 ± 0.03 | 0.45 ± 0.035 | 0.47 ± 0.029 | 0.67 ± 0.027* | 0.35 ± 0.014** | 0.48 ± 0.03*** |
CHAT protein | 0.085 ± 0.087 | 0.174 ± 0.013 | 0.174 ± 0.01 | 0.173 ± 0.011 | 0.167 ± 0.011** | 0.179 ± 0.09 | |
TH/CHAT | 2.18 ± 0.25 | 2.63 ± 0.26 | 2.67 ± 0.19 | 3.77 ± 0.20* | 2.66 ± 0.18** | 2.10 ± 0.10*** | |
4- HPLC (pmol/mg) | NE | 2.1 ± 0.12 | 4.3 ± 0.29 | 4.1 ± 0.31 | 5.1 ± 0.27* | 2.9 ± 0.26** | 3.9 ± 0.27*** |
ACh | 2.9 ± 0.26 | 3.9 ± 0.23 | 3.8 ± 0.27 | 3.5 ± 0.24 | 3.6 ± 0.28**** | 3.8 ± 0.27 |