Fig 4

HG-induced differentiation of mCFs was attenuated by resveratrol and NAC. a Effect of different glucose concentrations on the expression of α-SMA in mCFs. b Effect of different glucose concentrations on the expression of collagen I in mCFs. c Resveratrol attenuated upregulated α-SMA expression induced by HG in a dose dependent manner. d NAC mimicked the effect of resveratrol on expression of α-SMA induced by HG. e Immunofluorescence assay showed inhibition of ROS by resveratrol and NAC (E1, control group; E2, HG group; E3, HG + Res group; E4, HG + NAC group; E5, HG + Res + NAC group). f Upregulated α-SMA expression induced by HG was attenuated by resveratrol and NAC using immunofluorescence assay(F1, control group; F2, HG group; F3, HG + Res group; F4, HG + NAC group; F5, HG + Res + NAC group). * p < 0.05 between two groups; **p < 0.01 between two groups; bar = 50 μm